Data/hora: 20/05/2024 09:14:48

Biblioteca(s): 	Epagri-Sede.	
Tipo da produção científica: 	Resumo em Anais de Congresso	
Data corrente: 	24/10/2016	
Data da última atualização: 	24/10/2016	
Autoria: 	MERLIN, N.; KARLING, M.; MORALES, R. G. F.; OLDONI, T. L. C.	
Título: 	Profile of Phenolic Antioxidants from Moringa oleifera Leaves.	
Ano de publicação: 	2016	
Fonte/Imprenta: 	In: BRAZILIAN CONGRESS OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 48., LATIN AMERICAN CONGRESS OF PHARMACOLOGY, 21., 2016, Foz do Iguaçu, PR. Resumos... Curitiba, PR: SBFTE, 2016.	
Idioma: 	Inglês	
Conteúdo: 	Introduction: Moringa oleifera leaves have a wide variety of phenolic compounds, especially flavonoids [1, 2]. Since there are few investigations on the antioxidant activity (AA) of M. oleifera grown in Brazil, the aim of the present study was to evaluate the profile of phenolic compounds from leaves extracts of this species, collected in Itajaí ? Santa Catarina, by HPLC-DAD, and its AA, by an in vitro assay. Methods: Plant identification was performed and a voucher specimen (HPB 483) was deposited at the Herbarium of UTFPR, campus Pato Branco. M. oleifera leaves were dried in an oven at 40-45 °C and were ground. Successive extraction was carried out at room temperature, under shaking. Solvents were added in the following order: hexane (E-Hex), dichloromethane (E-CH2Cl2), ethyl acetate (E-EtOAc), acetone (E-Ace), ethanol (E-EtOH) and 50% ethanol (E-Hydro). Each solvent was replaced four times. Extracts were evaporated in a rotary evaporator and E-Hydro was also lyophilized. AA was determined, in triplicate, by the ABTS free radical scavenging method [3]. The determination of phenolic profile was performed on a Varian 920 liquid chromatograph, using a C18 Agilent column (250 x 4,6 mm, 5 µm), wich was maintained at 30 °C, and a diode array detector. 10 µL of extracts, prepared in a concentration of 15 g L-1, were injected. The mobile phase was a mixture of water:acetic acid (98:2, v/v) (solvent A) and acetonitrile:water:acetic acid (40:58:2, v/v) (solvent B), with a flow rate of 1 mL min-1, in a gradient mode. Identification was made by comparison of the absorption spectrum in the ultraviolet region and of the retention time with standards of phenolic acids: gallic, vanillic, caffeic, coumaric, ferulic and salicylic; flavonoids: catechin, epicatechin, rutin, myricetin and quercetin; and the stilbene trans-resveratrol. Results: Five of the phenolic standards were identified in at least one of the extracts: two monophenols, gallic and caffeic acids; and three flavonoids, epicatechin, rutin and quercetin. The lowest AA were observed for E-Hex and E-CH2Cl2 (149,04 ± 15,03 and 191,34 ± 5,33 µmol Trolox/g extract, respectively), results that should be related to the chromatographic profiles shown by both extracts, with few low intensity signals. On the other side, E-Hydro showed the best AA (789,54 ± 45,74 µmol Trolox/g extract) and, also, a profile with high intensity signals. Moreover, the diversity of chromatographic signals observed for E-EtOAc, E-Ace, E-EtOH and E-Hydro suggests that M. oleifera leaves have various bioactive compounds. Conclusion: Extracts showed distinct chemical profiles, proving the extraction capacity of solvents with different polarities. Together, the AA and the chromatographic profiles of E-EtOAc, E-Ace, E-EtOH and E-Hydro suggest the potential of M. oleifera leaves, grown in Itajaí, as a source of bioactive molecules.	
Palavras-Chave: 	antioxidant activity; flavonoids; phenolic compounds.	
Categoria do assunto: 	F Plantas e Produtos de Origem Vegetal	
URL: 	http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_38504.pdf	
Registro original: 	Epagri-Sede (Epagri-Sede)	
Exemplares: 	Epagri-Sede - (100682-1) - UPC - PL - DD -  -  -  - DIS